Publications by authors named "Abhijeet A Patil"
Article Synopsis
- The study focuses on the role of G-quadruplex (G4) DNA structures, formed by noncanonical base pairing among guanines, and their impact on gene expression in neurons.
- Researchers used a ligand called pyridostatin (PDS) to stabilize G4 structures, which led to the discovery of 901 differentially expressed genes in neurons, affecting crucial processes like p53 signaling and memory functions.
- The findings highlight a new mechanism involving the E3 ubiquitin ligase Pirh2, which is linked to DNA damage responses and increased G4-DNA levels, suggesting that G4 stabilization may influence both gene regulation and DNA integrity in neurons.
Tardigrades are microscopic invertebrates, which are capable of withstanding extreme environmental conditions, including high levels of radiation. A Tardigrade protein, Dsup (Damage Suppressor), protects the Tardigrade's DNA during harsh environmental stress and X-rays. When expressed in cancer cells, Dsup protects DNA from single- and double-strand breaks (DSBs) induced by radiation, increases survival of irradiated cells, and protects DNA from reactive oxygen species.
View Article and Find Full Text PDFSodium dodecyl sulfate-polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment. Our laboratory uses drying frames from RPI, which recommends a drying solution of 20% ethanol and 10% glycerol.
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