Influence of intercellular agents on proliferation and gene activity of cultured human skin epithelium cells (NCTC 2544).

"Human skin epithelium cells" (NCTC strain 2544; HSEpicell) are established cells and grow to a monolayer the same way as epithelial cells. Addition of proliferating or antiproliferating substances results in a typical dose-dependent influence on the cell growth: steroids inhibit mitosis, polyamines stimulate proliferation, while prostaglandin E2, theophylline and papaverine reduce cell growth. Since the pattern of chromosomal nonhistone proteins indicates alterations of gene activity, DNA-binding proteins of HSE picells are analysed.

Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). II. Viscosity changes after enzyme treatment.

Human skin epithelial-like cells (NCTC strain 2544) were grown in NCTC 135 medium. Neuraminidase and hyaluronidase were added to the growth medium. Cells were incubated 96 h at 36 degrees C.

Disc-electrophoretic separation of water soluble proteins from scales of different forms of ichthyosis.

In extracts of scales of different forms of ichthyosis, disc-electrophoretic separation of water soluble proteins was performed. Number and position of the protein bands correspond with number and position of the bands of extracts of normal keratin and psoriatic scales. However, in comparison to normal keratin, all examined forms of ichthyosis showed in zone II the enriched bands Nos.

Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). I. Measurement of viscosity.

Viscosities of cell suspensions of human skin epithelium (NCTC strain 2544) were determined by a Wells-Brookfield rotation viscosimeter. A structure viscosity may be postulated by the form of the viscosity curves at different shear-rates. At a cell number of 0.