Automated pixel-based quantification of porcine circovirus 2 genome in formalin-fixed, paraffin-embedded tissues using hybridisation.

Introduction: Detection of porcine circovirus 2 (PCV2) in lymphoid tissues is essential for diagnostic and research purposes. hybridisation (ISH) enables the localisation of viral genomes in tissue sections but is traditionally assessed visually, which may introduce subjectivity.

Methods: This study developed an automated pixel classifier to quantify the PCV2 genome using RNAscope ISH technology.

Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows.

Background/objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV.

Methods: This study evaluates the early protective efficacy of FlagT4G, a novel live attenuated DIVA-compatible vaccine. Pregnant sows were vaccinated at mid-gestation and challenged 14 days later with a highly virulent CSFV strain.

Pyrrolizidine alkaloid intoxication outbreaks in fattening pigs associated with drought-related feed contamination.

Background: Pyrrolizidine alkaloid (PA) intoxication is a well-documented condition in livestock, resulting from the ingestion of forage and grain contaminated with PA-producing plants. These phytotoxins primarily affect the liver and can lead to severe clinical and pathological disorders, particularly in highly susceptibility species such as pigs. Although sporadic cases of chronic PA toxicosis have been reported in swine, extensive outbreaks affecting large geographic areas have not been previously documented.

Review of porcine circovirus 3-associated lesions in swine: Challenges and advances in diagnostics.

Porcine circovirus 3 (PCV3) is a recently identified pathogen in swine populations. It is considered a ubiquitous virus and is frequently associated with subclinical infections throughout various stages of production. PCV3 is detectable in diverse tissues, blood, and secretions, indicating systemic dissemination and potential for both vertical and horizontal transmission.

Geographically structured genetic and morphological variation in a new species of (Squamata, Gekkonidae) from a karstic archipelago in western Cambodia.

A new species of karst-dwelling Bent-toed Gecko (genus ) is described from an unexplored karstic archipelago in western Cambodia. is composed of four allopatric, monophyletic mitochondrial lineages based on the ND2 gene. All are statistically diagnosable from one another based on univariate (ANOVA) and multivariate (PCA, DAPC, and MFA) analyses using a suite of size-corrected morphometric, meristic, and categorical color pattern and morphological characters.