98%
921
2 minutes
20
The Lactobacillus helveticus OSU-PECh-4A strain, from the Ohio State University Parker Chair collection, produces exceptional β-galactosidase activity using acid whey as a culture medium, compared with a commercial broth. The strain has a genome sequence of 1,834,843 bp, and its GC content is 36.69%. Using InterProScan v5.50-84.0 software, four genes with putative β-galactosidase function were found.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759395 | PMC |
http://dx.doi.org/10.1128/MRA.00766-21 | DOI Listing |
Genes Brain Behav
October 2025
Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Major depressive disorder is a prevalent and debilitating psychiatric illness that produces significant disability. Clinical data suggest that the pathophysiology of depression is due, in part, to a dysregulation of inflammation and glutamate levels in the brain. The systemic administration of lipopolysaccharide (LPS) has been shown to induce depressive-like behaviors in mice.
View Article and Find Full Text PDFJ Neural Transm (Vienna)
September 2025
Sárospatak College, Sztárai Institute, University of Tokaj, Eötvöst str. 7, Sárospatak, 3944, Hungary.
Generalized Anxiety Disorder (GAD) is characterized by excessive worry and physical symptoms of prolonged anxiety. Patients with subclinical GAD-states (sub-GAD) do not fulfill the diagnostic criteria of GAD, but they often show a disease burden similar to GAD, and the subclinical state may turn into a full syndrome. Neuroinflammation may contribute to changes in brain structures in sub-GAD, but direct evidence remains lacking.
View Article and Find Full Text PDFJ Neurosci
September 2025
Psychiatry, University of Minnesota, Minneapolis, Minnesota 55455
Deep brain stimulation (DBS) is an emerging treatment for otherwise treatment-refractory psychiatric disorders. It can produce remarkable clinical results in expert hands, but has not fared as well in controlled, multisite trials. That difficulty with scaling up arises in part because DBS' mechanisms are poorly understood, meaning that it is difficult to objectively identify patients likely to respond and/or to customize stimulation to match individual patients' needs.
View Article and Find Full Text PDFBiochim Biophys Acta Biomembr
September 2025
Department of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna, Austria.. Electronic address:
Using the stable synthetic analogue 3-aza-dehydroxylysyl-phosphatidylglycerol (3adLPG), the putative role of native staphylococcal LPG in inhibiting the antibiotic daptomycin from binding to its target phosphatidylglycerol (PG), was investigated with respect to interfacial interactions between these lipids, daptomycin, and calcium ions. The influence of lipid monolayer/bilayer composition and interfacial ion concentrations upon the structure and integrity of model membranes were probed after daptomycin challenge using a combination of surface x-ray scattering techniques and fluorescence assays. In models representing the membrane composition of the daptomycin susceptible phenotype consisting of PG/3adLPG in a 7:3 M ratio, calcium ions drive the formation of two separate phases; Ca cross-linked PG/PG pairs and PG/3adLPG ion pairs.
View Article and Find Full Text PDFGenomics
September 2025
Laboratory of Single Cell Analyses, Institute of Bioorganic Chemistry Polish Academy of Sciences, Zygmunta Noskowskiego str. 12/14, 61-704 Poznań, Poland. Electronic address:
Despite advancements in genome annotation tools, challenges persist for non-classical model organisms with limited genomic resources, such as Schmidtea mediterranea. To address these challenges, we developed a flexible and scalable genome annotation pipeline that integrates short-read (Illumina) and long-read (PacBio) sequencing technologies. The pipeline combines reference-based and de novo assembly methods, effectively handling genomic variability and alternative splicing events.
View Article and Find Full Text PDF