Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12010-019-03195-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
AFM-Based Monitoring of Enzymatic Activity of Individual Molecules of Cytochrome CYP102A1.
Biosensors (Basel)
May 2025
Institute of Biomedical Chemistry, 10, Pogodinskaya St., Moscow 119121, Russia.
Herein, we report the use of a nanotechnology-based approach for the study of enzyme-functionalized mica surfaces. Atomic force microscopy (AFM) has been employed for the determination of the catalytic activity of single molecules of heme-containing cytochrome P450 CYP102A1 (CYP102A1) enzyme, which was immobilized on the surface of a mica chip. Height fluctuations in individual molecules of the enzyme were measured under near-native conditions by AFM measurements in liquid using a cantilever with a 10 to 20 nm tip curvature radius.
View Article and Find Full Text PDFTyrosinase-based nanobiosensor for environmental monitoring of hormones in river water.
Environ Res
June 2024
Department of Food Engineering, URI, Erechim, Av. Sete de Setembro, 1621, 99709-910, Erechim-RS, Brazil. Electronic address:
This study explores the application of a tyrosinase cantilever nanobiosensor for detecting 17β-estradiol and estrone in typical water systems. The physical-chemical parameters of water were evaluated within the Tigre River micro-basin in Erechim, RS, to determine water potability for urban populations. Water clarity, conductivity, and pH levels were essential markers, adhering to recognized standards for water quality and human consumption.
View Article and Find Full Text PDFA perspective view on the nanomotion detection of living organisms and its features.
J Mol Recognit
December 2020
Laboratoire de Physique de la Matière Vivante, Institut de Physique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die.
View Article and Find Full Text PDFCantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water.
Appl Biochem Biotechnol
April 2020
Department of Food Engineering, URI Erechim, Av. Sete de Setembro, 1621, Erechim, RS, 99709-910, Brazil.
This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response.
View Article and Find Full Text PDFHeavy metals detection in river water with cantilever nanobiosensor.
J Environ Sci Health B
April 2020
Department of Food Engineering, URI - Erechim, Erechim, RS, Brazil.
Heavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water.
View Article and Find Full Text PDF