CRISPR/Cas9-engineering of Kell null erythrocytes to unveil host targeted irresistible antimalarial.

Malaria elimination faces challenges from drug resistance, stemming from mutations within the parasite's genetic makeup. Genetic adaptations in key erythrocyte proteins offer malaria protection in endemic regions. Emulating nature's approach, and implementing methodologies to render indispensable host proteins inactive, holds the potential to reshape antimalarial therapy.

Anti-retroviral therapy adherence in India (2012-18): A systematic review and meta-analysis.

Background: India has 2.1 million people living with HIV/AIDS (PLHIV). The objective of this study was to ascertain the extent of anti-retroviral therapy (ART) adherence and reasons for nonadherence among PLHIV in India.

Development of pathophysiologically relevant models of sickle cell disease and β-thalassemia for therapeutic studies.

Ex vivo cellular system that accurately replicates sickle cell disease and β-thalassemia characteristics is a highly sought-after goal in the field of erythroid biology. In this study, we present the generation of erythroid progenitor lines with sickle cell disease and β-thalassemia mutation using CRISPR/Cas9. The disease cellular models exhibit similar differentiation profiles, globin expression and proteome dynamics as patient-derived hematopoietic stem/progenitor cells.

Biosorption of halophilic fungal melanized membrane - PUR/melanin polymer for heavy metal detoxification with electrospinning technology.

Eradication of heavy metal pollution has become the prime priority over the conservation of water resources in the upcoming era. Herein, the study involved the halophilic fungal melanin from showed a promising biosorbent for the removal of toxic heavy metals which shows eco-friendly, cost-effective, high stability, and adsorbent efficiency. Polyurethane blended with fungal melanin polymers, makes polymeric nanofibrous membranes through electrospinning techniques.

Scalable noninvasive amplicon-based precision sequencing (SNAPseq) for genetic diagnosis and screening of β-thalassemia and sickle cell disease using a next-generation sequencing platform.

β-hemoglobinopathies such as β-thalassemia (BT) and Sickle cell disease (SCD) are inherited monogenic blood disorders with significant global burden. Hence, early and affordable diagnosis can alleviate morbidity and reduce mortality given the lack of effective cure. Currently, Sanger sequencing is considered to be the gold standard genetic test for BT and SCD, but it has a very low throughput requiring multiple amplicons and more sequencing reactions to cover the entire HBB gene.