High-Yield Convenient Mass Production of High-Quality Homogenous Human Induced Pluripotent Stem Cell Spheroids under Rho-Associated Kinase Inhibitor-Free 3D Culture Enabled by Micropatterning and Cold-Triggered Chemical-Free Cell Detachment.

Human induced pluripotent stem cells (hiPSCs) show great promise for personalized cell-based medicine, as they can be derived from easily accessible somatic cells and differentiated into all three germ layers without ethical concerns. This requires mass production of hiPSCs in 3D. However, contemporary methods for 3D culture result in hiPSC spheroids with significant size heterogeneity that is undesired for controlled differentiation and require the use of a high concentration of Rho-associated kinase inhibitor (RI) to improve the cell viability.

Student and Staff Knowledge of School Tobacco Policies and Student E-Cigarette Use, California 2022-2023.

Background: Since 2016, California has prohibited e-cigarette use alongside smoking in K-12 schools. School tobacco policies may reduce student e-cigarette use.

Methods: We analyzed 2022 to 2023 California Healthy Kids Survey (CHKS) and School Staff Survey data (n = 219,114).

Effectiveness of silver dihydrogen citrate (SDC) and chlorine dioxide gas (ClO) as sanitation strategies for picking bags and storage bins at tree fruit production facilities.

Foodborne outbreaks and recalls within the tree fruit industry are making producers re-evaluate appropriate cleaning and sanitation practices during harvesting. Without effective sanitation, bacteria can create niches and form biofilms. This study evaluated the efficacy of silver dihydrogen citrate (SDC) and chlorine dioxide (ClO) gas to control Escherichia coli and Listeria innocua on experimentally inoculated harvesting equipment at commercial apple packinghouses within the Midwest and Pacific Northwest regions.

Symptoms matter - symptom diversity and trajectory across different phases of heart failure: from diagnosis to end of life.

Purpose Of Review: Heart failure is a complex, progressive and life-limiting condition that affects individuals beyond physical symptoms. Psychosocial and behavioural symptoms, such as anxiety, depression, cognitive impairment, and social withdrawal, substantially impact their quality of life. Despite increasing recognition of multidimensional symptom burden across heart failure trajectory, these non-physical symptoms are often under-recognised by clinicians and researchers.

Executive control in Obsessive-Compulsive Disorder: A worldwide mega-analysis of task-based functional neuroimaging data of the ENIGMA-OCD consortium.

Objective: Obsessive-compulsive disorder (OCD) is associated with impaired executive function and altered activity in associated neural circuits, contributing to reduced goal-directed behavior. To investigate neural activation during executive control, we conducted a mega-analysis in the ENIGMA-OCD consortium pooling individual participant data from 475 individuals with OCD and 345 healthy controls across 15 fMRI tasks collected worldwide.

Methods: Individual participant data was uniformly processed using HALFpipe to construct voxelwise statistical images of executive control and task load contrasts.