Proviral functions of HMGB1 in HAdV-C5 replication compartments.

Unlabelled: Human adenoviruses (HAdVs) induce significant reorganization of the nuclear environment, leading to the formation of virus-induced subnuclear structures known as replication compartments (RCs). Within these RCs, viral genome replication, gene expression, and modulation of cellular antiviral responses are tightly coordinated, making them valuable models for studying virus-host interactions. In a recent study, we analyzed the protein composition of HAdV type 5 (HAdV-C5) RCs isolated from infected primary cells at different time points during infection using quantitative proteomics.

Design and in vitro evaluation of thiolated alginates with N-acetyl-L-cysteine/L-cysteine substructures.

A new generation S-protected thiolated alginate was synthesized using a ligand based on l-cysteine ​​protected with N-acetyl-l-cysteine (NACys-Cys) ​​in order to obtain a thiomer with improved mucoadhesive properties. Two types of alginate with different molar mass were evaluated with two ligand concentrations. The successful protection with NACys-Cys was confirmed with FT-IR, NMR and free amino group quantification.

Percutaneous or surgical revascularization in patients with severe left main coronary artery disease in Latin America: A GRADE clinical practice guideline.

Background: Severe left main coronary artery disease (LMD) poses a major treatment challenge in Latin America, where both percutaneous coronary intervention (PCI) and coronary artery bypass grafting (CABG) are used.

Methods: This guideline was developed de novo using the GRADE approach. A multidisciplinary panel reviewed evidence from a systematic review of randomized trials comparing PCI and CABG, incorporating a comprehensive literature search of patient values and preferences and outcome utilities.

Mapping the interaction surface between Caβ and actin and its role in calcium channel clearance.

Defective ion channel turnover and clearance of damaged proteins are associated with aging and neurodegeneration. The L-type Ca1.2 voltage-gated calcium channel mediates depolarization-induced calcium signals in heart and brain.