Structural basis of poxvirus fusion regulation and anti-A16/G9 antibody-mediated neutralization and protection.

Monkeypox virus (MPXV) is a poxvirus endemic to Central and West Africa with high epidemic potential. Poxviruses enter host cells via a conserved entry-fusion complex (EFC), which mediates viral fusion to the cell membrane. The EFC is a promising therapeutic target, but the absence of structural data has limited the development of fusion-inhibiting treatments.

TLR7/8 ligands R848 and imiquimod induce differentiation of bone marrow cells from patients with myelodysplastic syndrome towards mature neutrophils.

Myelodysplastic syndromes (MDS) and acute myeloid leukaemia (AML) arise as a consequence of acquisition and progressive accumulation of genetic and epigenetic modifications by haematopoietic stem and progenitor cells (HSPC) which result in an impaired cell differentiation and the clonal expansion of myeloid progenitors leading to blast-cell accumulation in bone marrow (BM) and myelodysplasia. TLRs are expressed on HSPC and play a role in modulating haematopoiesis by instructing commitment to the myeloid lineage, and therefore may have potential therapeutic application. We have determined the in vitro effect of R848 (TLR7/TLR8 agonist) and Imiquimod (TLR7 agonist), on differentiation, apoptosis and cell viability in primary cultures of bone marrow samples from MDS (n = 6) and AML patients (n = 13).

Pelvic Floor Adaptation to a Prenatal Exercise Program: Does It Affect Labor Outcomes or Levator Ani Muscle Injury? A Randomized Controlled Trial.

Physical exercise during pregnancy is strongly recommended due to its well-established benefits for both mother and child. However, its impact on the pelvic floor remains insufficiently studied. This study aimed to evaluate pelvic floor adaptations to a structured prenatal exercise program using transperineal ultrasound, and to assess associations with the duration of the second stage of labor and mode of delivery.

Reproducibility of Breech Progression Angle: Standardization of Transperineal Measurements and Development of Image-Based Checklist for Quality Control.

To evaluate the reproducibility of measurements of breech progression angle (BPA) by transperineal ultrasound (US) before and after its standardization by applying an image-based checklist. Eighteen 3-dimensional (3D) volumes of transperineal US from women at 36-40 weeks of gestation with a singleton fetus in breech presentation were provided to eight operators from four maternity units in Spain and Italy. All operators measured the BPA using 3D US volume processing software, and interobserver reproducibility was evaluated using the intraclass correlation coefficient (ICC).

Placental growth factor before 11 weeks for screening of preterm preeclampsia: The PreMoM study.

Introduction: Our objective was to compare the predictive performance of the Fetal Medicine Foundation (FMF) competing-risk model for preterm preeclampsia (PE) screening using placental growth factor (PlGF) measurements obtained at 11-13 weeks versus before 11 weeks of gestation.

Material And Methods: This multicenter prospective cohort study included women with singleton pregnancies attending their routine first-trimester assessment (11 to 13 weeks) in four hospitals across Spain from 2021 to 2023. Maternal characteristics, biophysical parameters (mean arterial pressure and uterine artery pulsatility index), and biochemical markers (PlGF measured twice in each woman, before 11 weeks and between 11 and 13 weeks) were assessed.