Functional Integration of Different-Sex Gonad Transplants into the Adult Mouse Hypothalamic Pituitary Gonadal Axis.

Gender-affirming hormone therapy (GAHT) relies on exogenous hormones to produce hormonal milieus that achieve and/or maintain embodiment goals. Another potential route to these endpoints is transplantation of novel steroidogenic tissue. To develop a pre-clinical model, we asked whether different-sex gonad transplants can be functionally integrated into the adult mouse hypothalamic-pituitary-gonadal (HPG) axis.

Remote ischemic conditioning-induced shift from a vulnerable to a tolerant penumbra: A proteomic perspective.

The concept of the ischaemic penumbra - stroke tissue with the potential to survive - has opened the door to a wide range of experimental strategies that could benefit the recovery of patients after a stroke. In this study, we used proteomic analysis to examine how remote ischaemic postconditioning (RIPC) mediates a shift from a vulnerable to a tolerant penumbra. We identified 450 differentially abundant proteins between the control group and the groups subjected to ischaemia via middle cerebral artery occlusion with or without RIPC during infarct expansion.

Functional changes to Achilles tendon and enthesis in an adolescent mouse model of testosterone hormone therapy.

Purpose/aim: Some youth seek puberty suppression to prolong decision-making prior to starting hormone therapy to help align their physical sex characteristics with their gender identity. During peripubertal growth, connective tissues such as tendon rapidly adapt to applied mechanical loads (e.g.

Short and long duration testosterone treatments induce reversable subfertility in female mice using a gestational model of gender-affirming hormone therapy.

Study Question: How does testosterone gender-affirming hormone therapy (T-GAHT) impact breeding success in female mice?

Summary Answer: T-GAHT causes reversible subfertility in female mice and persistent changes to reproductive tract anatomy, gene expression, and hormone receptors.

What Is Known Already: Adult female mice implanted with capsules containing 10 mg of testosterone mimic many aspects of reproductive phenotypes of T-GAHT patients, who may desire future gestation while pausing T-GAHT. In mice, oocytes retrieved from T-GAHT mice had decreased IVF rates, and T cessation prior to stimulation improved these outcomes.

Enhancing the prediction of arsenic bioavailability in soils with the diffusive gradient in thin film technique.

The diffusive gradient in thin films technique (DGT), with a resin gel based on Lewatit® FO 36 was used for the first time to predict arsenic (As) bioavailability in soils collected in different environmental contexts. The predicted bioavailability, determined by fluxes to DGT, was compared with the bioavailability and bioaccumulation in the plants (Calendula officinalis), where a strong correlation was observed (r = 0.8857 (C/C) and r = 0.