Anchored screening identifies transcription factor blueprints underlying dendritic cell diversity and subset-specific anti-tumor immunity.

Transcription factor cooperation is essential for specifying the heterogeneous dendritic cell (DC) lineages that orchestrate adaptive immunity, yet how it drives subset diversification remains poorly understood. Here, we employed a sequential anchored screen of 70 transcription factors using direct cellular reprogramming to identify regulators that specify type 2 conventional DCs (cDC2s) and plasmacytoid DCs (pDCs). We identified PU.

c-Myc derived DNAzyme and its application for the detection of a novel probiotic bacteria L. fermentum using the peroxidase mimicking activity.

Catalytically active DNA molecules are called DNAzymes. Single-stranded guanine-rich DNA molecules that can fold into G-quadruplex structures, on interaction with hemin, can exhibit peroxidase-like activity. In this study, we report a c-Myc G-quadruplex forming DNA sequence in complex with hemin, which demonstrates DNAzyme properties and aim to develop a rapid, simple, cost-effective, and culture-free method for detecting probiotic bacteria.

Recent Advancements in Immunotherapy for the Treatment of Metastatic Breast Cancer.

Breast cancer (BC) is the most prevalent malignancy among women in the United States, affecting approximately 13% of the female population. While advancements in treatment strategies have improved survival rates, significant challenges remain due to tumor heterogeneity, metastatic progression, and acquired resistance to therapy. Recent studies have highlighted the potential of immunotherapy in managing various solid tumors, including BC.

A proinflammatory immune response underlies impairment of intestinal barrier function in Holstein calves exposed to heat stress.

Heat stress has been associated with promoting a systemic and local proinflammatory response, which could involve impaired intestinal structure and function in growing dairy calves. We hypothesized that heat stress-induced reduction of the intestinal barrier function would be associated with changes in immune cell counts, and the administration of the glucocorticoid dexamethasone would attenuate the proinflammatory response and restore intestinal barrier function. The objective of the study was to determine the effect of reducing the proinflammatory response on systemic and local immune cell concentrations, focusing on the major leukocyte types (neutrophils and lymphocytes), as well as structural and functional changes in the intestine of dairy calves exposed to heat stress.