Rupture of the diaphragm caused by blunt trauma. Review of 54 successive cases.

From 1970 to 1987, 54 patients were treated for rupture of the diaphragm as a result of blunt trauma, mostly due to road accidents. In more than 75% of the cases, other severe post-traumatic lesions were noted in association with the rupture. There were 46 lesions in the left hemidiaphragm.

Parathyroid hormone-induced ornithine decarboxylase activity in fetal rat osteoblasts.

Induction of ornithine decarboxylase (ODC, E.C. 4.

Traumatic rupture of the diaphragm. Review of 62 successive cases.

From 1970 to 1987, 62 patients, suffering from traumatic rupture of the diaphragm, have been treated. In more than 75% of the cases, other severe post-traumatic associated lesions were noticed. In 54 cases these concerned blunt trauma, most of which were due to road accidents.

Modulatory function of protein kinase C in the activation of ornithine decarboxylase and in cAMP production in rat osteoblasts.

The effect of activation of protein kinase C on stimulation of ornithine decarboxylase (ODC) activity and cAMP production was studied in fetal rat osteoblasts. Both phorbol 12-myristate, 13-acetate (PMA), an activator of protein kinase C, and 4 alpha-phorbol, ineffective in activating protein kinase C, failed to stimulate ODC activity and cAMP production. We tested the effect of protein kinase C on stimulation of ODC activity by parathyroid hormone (PTH) and forskolin.

An experimental setup for the measurement of forces on a human cadaveric foot during inversion.

An experimental setup was developed for statically measuring seven vertical and three horizontal reaction forces on the foot. In the setup, the leg can be simultaneously loaded (1) by a vertical force, (2) by an externally applied axial moment, and (3) by simulated muscle forces. The foot is free to invert under influence of the external loads.

A model study of muscle forces and joint-force direction in normal and dysplastic neonatal hips.

Orthopaedic treatment of congenital hip dysplasia does not always give the desired result. With the present model, prediction of the effects of various treatments on the force direction in the hip joint could help to improve and select treatment (the force direction is presumed to control the collum growth direction). The model contains three-dimensional mathematical descriptions of all muscles passing the hip joint, for various degrees of femoral dysplasia, and for various hip postures.

[Trends in research related to the health of pigs].

The intensification of pig husbandry has important consequences for pig health care. More attention must not only be paid to clinical outbreaks of disease but also to subclinical problems and disturbances of production. More research must be done to improve the efficiency of disease control and beyond this to the optimalization of prevention.

Monoclonal antibodies to the major feline allergen Fel d I. I. Serologic and biologic activity of affinity-purified Fel d I and of Fel d I-depleted extract.

Monoclonal antibodies were raised against a major feline allergen, Fel d I. The specificity of the antibodies for Fel d I was demonstrated by a modified crossed immunoelectrophoretic procedure. These antibodies were used in affinity purification and depletion.

Heterologous up-regulation of the 1,25-dihydroxyvitamin D3 receptor by parathyroid hormone (PTH) and PTH-like peptide in osteoblast-like cells.

1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) receptor content in cultured osteogenic sarcoma cells (UMR-106) was found to be increased after treatment with both bovine and human PTH and human PTH-like peptide (hPLP). The dose dependent increase of receptors was preceded by a dose dependent stimulation of cAMP production. This suggests a role for cAMP as mediator of the PTH- and hPLP-induced 1,25-(OH)2D3 receptor up-regulation.

Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts.

The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187.

Induction of ornithine decarboxylase activity in isolated chicken osteoblasts by parathyroid hormone: the role of cAMP and calcium.

We investigated the role of cAMP and Ca2+ as mediators in parathyroid hormone (PTH)-induced ornithine decarboxylase (ODC) activity in primary cultures of chicken osteoblasts. We present evidence that the induction of ODC activity by PTH is most likely a receptor-mediated process and that cAMP is a mediator. However, using three different approaches we have strong indications that cAMP is not the exclusive mediator of PTH-induced ODC activity.

Effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblast cell line.

In the present study the effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblastic cell line (UMR 106-01) is examined. In these cells the intact hormone, bPTH(1-84) and three fragments (bPTH(1-34), hPTH(1-34) and bPTH(3-34) induce an initial increase of the ionized calcium concentration [( Ca2+]i). The increase of the [Ca2+]i due to bPTH(1-84) is more pronounced and of longer duration compared to the increase due to PTH fragments.

Involvement of cAMP and calcium in the induction of ornithine decarboxylase activity in an osteoblast cell line.

The role of cAMP and calcium in the induction of ornithine decarboxylase (ODC, E.C.4.

Different roles for calcium and cyclic AMP in the action of PTH: studies in bone explants and isolated bone cells.

In fetal mouse calvaria forskolin (0.1-100 microM), like PTH, stimulated cyclic AMP production in a dose-dependent way. The dose-response curve for forskolin-induced bone mineral release (24 hrs), however, demonstrated a biphasic character, showing stimulation at 0.

Axial rotation of murine embryos, a study of asymmetric mitotic activity in the neural tube of somite stages.

Axial rotation is an important event during a certain period of development of Amniote embryos. In murine embryos a sharp lordosis changes into a kyphosis. The result is the typical fetal position.

The value of target ultrasonic mammography and cytopathology in the assessment of round-shaped shadows on mammograms.

The preoperative value of target ultrasonic mammography (TUSM) and cytopathology, combined with clinical examination and mammography, was studied in 146 mammographically demonstrated round-shaped breast lesions. All lesions were biopsied and histology was performed. Advantages of combining TUSM with cytology are the possibility to have a more precise puncture aspiration with TUSM and a subsequently higher predictive value, compared to each technique alone.

A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP.

It has been suggested that intracellular Ca2+, in addition to cAMP, plays an important role in PTH-stimulated bone resorption. There is now strong evidence indicating that the osteoblast is the main target cell for PTH action, regulating indirectly, via cell-cell communication, osteoclastic bone resorption. In order to investigate the possible role of free cytosolic calcium in stimulated bone resorption, we studied the effects of the intact hormone (bPTH 1-84) and some of its fragments (bPTH (1-34), bPTH(3-34,) (Nle-8, Nle-18,Tyr-34) bPTH (3-34) amide) on their capacity to modify the cytosolic Ca2+ concentration in rat osteoblast-like cells.

The classification of Sejroe group serovars of Leptospira interrogans with monoclonal antibodies.

Using the hybridoma technique we produced monoclonal antibodies to serovars of Leptospira interrogans. We focussed on serovar hardjo which is an important pathogen for humans and animals, and on other serovars of the Sejroe group. With combinations of monoclonals, characteristic patterns of agglutination were observed according to the specific pattern of antigenic determinants of that serovar.

Ultrastructure of gap junctions in the central nervous system of Lymnaea stagnalis with particular reference to electrotonic coupling between the neuroendocrine caudodorsal cells.

Presence and structure of gap junctions have been studied at the ultrastructural level in the central nervous system of the freshwater snail Lymnaea stagnalis. Gap junctions are clearly visible in thin sections of glutaraldehyde-fixed and phosphotungstic acid-stained material as well as in freeze-fracture preparations. Various types can be distinguished on the basis of junctional size and shape, and numerical density, diameter, spacing and arrangement of junctional particles.

Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae.

Thirty-two monoclonal antibodies (MoAb) to Mycobacterium tuberculosis H37Rv, M. bovis BCG and M. leprae were produced.

A simple measurement hammer for quantitative reflex studies.

A reflex hammer for measurement of the mechanical stimulus strength was designed. Combined with standard EMG equipment this instrument permits the study of both stimulus-response relations and latencies of myotatic reflexes. Some results in normal subjects are discussed.

The importance of monopole-monopole and monopole-dipole interactions on the binding of NADPH and NADPH analogues to p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Effects of pH and ionic strength.

NADPH binding to p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens is found to be strongly dependent on pH and ionic strength. In the ionic strength range of 0.02-0.