USP37 protects mammalian cells during DNA replication stress by counteracting CUL2 and TRAIP.

The USP37 deubiquitylase is essential for mammalian cells to survive DNA replication stress, but the underlying mechanisms are unknown. Here, we demonstrate that USP37 binds the CDC45-MCM-GINS (CMG) helicase, which forms the stable core of the replisome until DNA replication termination when CMG is ubiquitylated and disassembled. USP37 contacts CDC45, and structure-guided mutations that displace USP37 from CMG cause sensitivity to DNA synthesis defects or topological stress.

Applications of Artificial Intelligence in Ophthalmology: Glaucoma, Cornea, and Oculoplastics.

Artificial intelligence (AI) is transforming ophthalmology by leveraging machine learning (ML) and deep learning (DL) techniques, particularly artificial neural networks (ANN) and convolutional neural networks (CNN) to mimic human brain functions and enhance accuracy through data exposure. These AI systems are particularly effective in analyzing ophthalmic images for early disease detection, improving diagnostic precision, streamlining clinical workflows, and ultimately enhancing patient outcomes. This study aims to explore the specific applications and impact of AI in the fields of glaucoma, corneal diseases, and oculoplastics.

A Review of the Utility and Limitations of Artificial Intelligence in Retinal Disorders and Pediatric Ophthalmology.

Artificial intelligence (AI) is reshaping ophthalmology by enhancing diagnostic precision and treatment strategies, particularly in retinal disorders and pediatric ophthalmology. This review examines AI's efficacy in diagnosing conditions such as diabetic retinopathy (DR) and age-related macular degeneration (AMD) using imaging techniques, such as optical coherence tomography (OCT) and fundus photography. AI also shows promise in pediatric care, aiding in the screening of retinopathy of prematurity (ROP) and the management of conditions, including pediatric cataracts and strabismus.

Promoting research integrity in funding: Co-creating guidelines for research funding organizations.

Research Funding Organizations (RFOs) play an important role in promoting research integrity (RI). Not only do they allocate resources to research institutions and researchers, but they also set and monitor research standards in their funded projects. In spite of their crucial role, there is a paucity of guidance on how RFOs can promote research integrity.

CMG helicase disassembly is essential and driven by two pathways in budding yeast.

The CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and disassembled during DNA replication termination. Fungi and animals use different enzymes to ubiquitylate the Mcm7 subunit of CMG, suggesting that CMG ubiquitylation arose repeatedly during eukaryotic evolution. Until now, it was unclear whether cells also have ubiquitin-independent pathways for helicase disassembly and whether CMG disassembly is essential for cell viability.