Probing the Role of Telopeptides in Collagen Fibrillogenesis by Second Harmonic Scattering.

Collagen, the primary structural protein in the extracellular matrix, plays a critical role in tissue architecture and mechanical integrity. This study investigates the structural and nonlinear optical properties of atelocollagen (Acol) and telocollagen (Tcol) in response to pH variations by using second harmonic scattering (SHS), polarization-resolved SHS (P-SHS), and atomic force microscopy (AFM). AFM imaging revealed distinct morphological differences, with Acol forming wavy, non-cross-linked, and randomly arranged fibers, while Tcol exhibited a more interconnected, mesh-like fibrillar network.

Warming of northern peatlands increases the global temperature overshoot challenge.

Meeting the Paris Agreement's temperature goals requires limiting future carbon emissions, yet current policies make temporarily overshooting the 1.5°C target likely. The potential climate feedback from destabilizing peatlands, storing large amounts of carbon, remains poorly quantified.

Independent validation and outlier analysis of EuroPOND alzheimer's disease staging model using ADNI and real-world clinical data.

Background: Event-based modeling (EBM) traces sequential progression of events in complex processes like neurodegenerative diseases, adept at handling uncertainties. This study validated an EBM for Alzheimer's disease (AD) staging designed by EuroPOND, an EU-funded Horizon 2020 project, using research and real-world datasets, a crucial step towards application in multi-center trials.

Methods: The training dataset comprised 1737 subjects from ADNI-1/GO/2, using the EuroPOND EBM toolbox.

[Evaluation of a female genital mutilation knowledge assessment tool].

Objective: This article aims to evaluate a questionnaire as a tool for assessing knowledge on female genital mutilation.

Materials And Methods: Prospective survey between November 2021 to May 2022. An anonymized questionnaire of 34 questions has been sent to residents of general medicine, forensic medicine, gynaecology and obstetrics and medical gynaecology.