Publications by authors named "D Holub"
Article Synopsis
- Laser-induced breakdown spectroscopy (LIBS) enhances immunochemical assays using indirect label detection with nanoparticles, specifically yttrium-based photon-upconversion nanoparticles (UCNPs), achieving sensitivity comparable to established methods like fluorescence immunoassays.
- The study improves the sensitivity of UCNP-based Tag-LIBS by incorporating magnetic microbeads (MBs), which allow for analyte preconcentration and significantly lowers the limit of detection (LOD) to 4.0 pg/mL, a notable advancement over traditional microtiter plate methods.
- The technique demonstrates robust performance in clinical samples and shows strong correlation with standard assays, indicating that MB-based Tag-LIBS offers fast readout, high sensitivity, and potential for multiplexing without relying
People are exposed to microplastics (MPs) on a large scale in everyday life. However, it is not clear whether MPs can also be distributed and retained in certain tissues. Therefore, the development of analytical methods capable of detecting MPs in specific human organs/tissues is of utmost importance.
View Article and Find Full Text PDFContamination of the environment with toxic metals such as cadmium or lead is a worldwide issue. The accumulator of metals Cannabis sativa L. has potential to be utilized in phytoremediation, which is an environmentally friendly way of soil decontamination.
View Article and Find Full Text PDFBackground: Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples or are present artificially.
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- Amyloidosis is a rare disorder caused by misfolded proteins, leading to abnormal deposits in tissues, including the lungs, where nodular pulmonary amyloidosis is particularly uncommon and often asymptomatic.* -
- The only documented case of nodular pulmonary amyloidosis causing clinical symptoms, like spontaneous pneumothorax, involved an 81-year-old male with an AH-dominant type, highlighting its rarity and complexity.* -
- Accurate identification of amyloid types is challenging but essential for understanding the underlying disease and determining appropriate diagnostic and treatment strategies, making it important in differential diagnoses of pulmonary conditions.*