Validation of uPath HER2 dual-colour dual in-situ hybridisation image analysis tool for HER2/neu testing in breast cancer.

Aims: gene is amplified in 15%-20% of invasive breast cancers (IBCs), serving as critical prognostic and predictive marker. -targeted therapies have improved outcomes for -positive patients, highlighting the importance of accurate assessment. Immunohistochemistry is commonly used for screening overexpression, with equivocal cases reflex tested using in situ hybridisation (ISH) methods like fluorescence (FISH) or dual-colour dual ISH (D-DISH).

Increase in productivity enhances CH but limits NO production in a shallow tropical lake experiencing seasonal volume reduction and salinization.

Lakes are hotspots for carbon and nitrogen cycling and a major contributor to the natural fluxes of greenhouse gases [GHGs: methane (CH), carbon dioxide (CO), and nitrous oxide (NO)] to the atmosphere. Lake volume reduction and salinization are major threats to lakes across the globe, and their effect on lake biogeochemistry warrants detailed investigation. Given the majority of the studies addressing this crisis are from the higher latitudes, the response of tropical lakes, and shallow lakes in particular, to changing magnitude of environmental stressors is a major research gap.

Analysis of an alternatively spliced variant of mouse Pklr gene encoding a novel isoform with distinct N-terminal.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate and ADP to pyruvate and ATP in glycolysis and plays a role in regulating cell metabolism. Mammalian pyruvate kinase functions as a tetrameric protein composed of identical subunits, which adopt a dimer-of-dimers configuration. Each monomer features a single active site and consists of three primary domains designated A, B and C, and a small N-terminal domain.

CHASHNIt for enhancing skin disease classification using GAN augmented hybrid model with LIME and SHAP based XAI heatmaps.

Correct categorization of skin diseases is vital for prompt diagnosis. However, obstacles such as imbalance of data and interpretability of deep learning models limit their use in medical settings. To overcome these setbacks, Combined Hybrid Architecture for Scalable High-performance in Neural Iterations or CHASHNIt is proposed, which is an integration of EfficientNetB7, DenseNet201, and InceptionResNetV2 to outperform current models on every ground.

Acute cutaneous adverse effects of methotrexate: A case report highlighting therapeutic challenges.

Methotrexate (MTX) is a commonly used antimetabolite for managing various autoimmune disorders and cancers. While it is generally well-tolerated at low doses, certain factors may predispose patients to significant toxicity. Here we present a case of 49-year-old male with advanced buccal mucosa squamous cell carcinoma who developed acute severe cutaneous toxicity following low dose of intravenous MTX therapy.